Acid Intracellular Vesicles and the Cytolysis of Mammalian Target Cells by Entamoeba histolytica Trophozoites
Identifieur interne : 003442 ( Main/Exploration ); précédent : 003441; suivant : 003443Acid Intracellular Vesicles and the Cytolysis of Mammalian Target Cells by Entamoeba histolytica Trophozoites
Auteurs : Jonathan I. Ravdin [États-Unis] ; Paul H. Schlesinger [États-Unis] ; Cheryl F. Murphy [États-Unis] ; Ilya Y. Gluzman [États-Unis] ; Donald J. Krogstad [États-Unis]Source :
- The Journal of Protozoology [ 0022-3921 ] ; 1986-11.
English descriptors
- Teeft :
- Acid vesicles, Ameba, Amebic, Amebic adherence, Amebic cytolysis, Amebic vesicle, Ammonium, Assay, Baseline, Capracanis, Cell monolayers, Chloroquine, Coli, Control medium, Control test medium, Cycloheximide, Cytochalasin, Cytolysis, Cytolytic, Cytotoxicity studies, Dextran, Endocytic vesicles, Entamoeba, Entamoeba histolytica, Glass chips, Histolytica, Human neutrophils, Intracellular, Lectin, Lysosomal, Lysosomal enzymes, Macrophage, Mammalian cells, Mammalian target cells, Meronts, Neutrophil, Parasite, Pellet, Phagocytic events, Preincubation, Primaquine, Protein synthesis, Ravdin, Receptor, Rosette, Standard curve, Supernatant fluid, Surface lectin, Target cells, Test conditions, Test medium, Trichloroacetic material, Trophozoite, Trypan, Vascular phase, Vesicle.
Abstract
ABSTRACT. Entamoeba histolytica kills mammalian target cells in a multi‐step sequential process with separate adherence, cytolytic, and phagocytic events. In the studies reported here, we used fluorescein isothiocyanate linked to dextran to label the endocytic vesicles of the HM1 strain of E. histolytica and measure vesicle pH (5.1 ± 0.2 by spectrofluorimetry). Concentrations of NH4Cl (1.0–10.0 mM) sufficient to increase vesicle pH to °5.7 inhibited amebic killing of target Chinese hamster ovary (CHO) cells as assayed by trypan blue staining, by the release of 3H‐thymidine previously incorporated into CHO cell monolayers, and by the release of 111indium oxine from radiolabeled CHO cells. Similar effects were also observed with two other weak bases, primaquine and chloroquine (both 50 μM). In contrast, NH4Cl (10 mM) did not affect either the adherence or phagocytic events, as measured by amebic adherence to CHO cells at 4°C and by the binding and ingestion of 3H‐leucine‐labeled bacteria. In the presence of NH4Cl and and the carbohydrate ligand asialofetuin, there was no evidence of intracellular trapping of the amebic galactose‐inhibitable lectin; inhibition of adherence by cycloheximide (10 μg/ml for 3 h) suggested rapid turnover of the surface lectin. Prolonged exposure to NH4Cl for 48 h (which had no effect on amebic protein synthesis) or shorter exposure to cycloheximide (10 μg for 3 h) produced persistent inhibition of cytolysis. These results indicate that an uninterrupted acid pH in intracellular endocytic vesicles is necessary for the cytolysis of target cells by E. histolytica trophozoites.
Url:
DOI: 10.1111/j.1550-7408.1986.tb05646.x
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000A64
- to stream Istex, to step Curation: 000A64
- to stream Istex, to step Checkpoint: 002201
- to stream Main, to step Merge: 003515
- to stream Main, to step Curation: 003442
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Acid Intracellular Vesicles and the Cytolysis of Mammalian Target Cells by Entamoeba histolytica Trophozoites</title><author><name sortKey="Ravdin, Jonathan I" sort="Ravdin, Jonathan I" uniqKey="Ravdin J" first="Jonathan I." last="Ravdin">Jonathan I. Ravdin</name></author><author><name sortKey="Schlesinger, Paul H" sort="Schlesinger, Paul H" uniqKey="Schlesinger P" first="Paul H." last="Schlesinger">Paul H. Schlesinger</name></author><author><name sortKey="Murphy, Cheryl F" sort="Murphy, Cheryl F" uniqKey="Murphy C" first="Cheryl F." last="Murphy">Cheryl F. Murphy</name></author><author><name sortKey="Gluzman, Ilya Y" sort="Gluzman, Ilya Y" uniqKey="Gluzman I" first="Ilya Y." last="Gluzman">Ilya Y. Gluzman</name></author><author><name sortKey="Krogstad, Donald J" sort="Krogstad, Donald J" uniqKey="Krogstad D" first="Donald J." last="Krogstad">Donald J. Krogstad</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:473566D72FFE98CCAD7462F82FCA9884F1ECC1D0</idno><date when="1986" year="1986">1986</date><idno type="doi">10.1111/j.1550-7408.1986.tb05646.x</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-GH0PSN0T-6/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000A64</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000A64</idno><idno type="wicri:Area/Istex/Curation">000A64</idno><idno type="wicri:Area/Istex/Checkpoint">002201</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">002201</idno><idno type="wicri:doubleKey">0022-3921:1986:Ravdin J:acid:intracellular:vesicles</idno><idno type="wicri:Area/Main/Merge">003515</idno><idno type="wicri:Area/Main/Curation">003442</idno><idno type="wicri:Area/Main/Exploration">003442</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Acid Intracellular Vesicles and the Cytolysis of Mammalian Target Cells by Entamoeba histolytica Trophozoites1</title><author><name sortKey="Ravdin, Jonathan I" sort="Ravdin, Jonathan I" uniqKey="Ravdin J" first="Jonathan I." last="Ravdin">Jonathan I. Ravdin</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Virginie</region></placeName><wicri:cityArea>Department of Internal Medicine, University of Virginia Medical Center, Charlottesville</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Virginie</region></placeName><wicri:cityArea>Correspondence address: Address preprint requests to Dr. Ravdin at the Division of Clinical Pharmacology, Box 419, Department of Internal Medicine, University of Virginia School of Medicine, Charlottesville</wicri:cityArea></affiliation></author><author><name sortKey="Schlesinger, Paul H" sort="Schlesinger, Paul H" uniqKey="Schlesinger P" first="Paul H." last="Schlesinger">Paul H. Schlesinger</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Missouri (État)</region></placeName><wicri:cityArea>Departments of Biological Chemistry, Washington University, St. Louis</wicri:cityArea></affiliation></author><author><name sortKey="Murphy, Cheryl F" sort="Murphy, Cheryl F" uniqKey="Murphy C" first="Cheryl F." last="Murphy">Cheryl F. Murphy</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Virginie</region></placeName><wicri:cityArea>Department of Internal Medicine, University of Virginia Medical Center, Charlottesville</wicri:cityArea></affiliation></author><author><name sortKey="Gluzman, Ilya Y" sort="Gluzman, Ilya Y" uniqKey="Gluzman I" first="Ilya Y." last="Gluzman">Ilya Y. Gluzman</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Missouri (État)</region></placeName><wicri:cityArea>Departments of Pathology and Medicine, Washington University, St. Louis</wicri:cityArea></affiliation></author><author><name sortKey="Krogstad, Donald J" sort="Krogstad, Donald J" uniqKey="Krogstad D" first="Donald J." last="Krogstad">Donald J. Krogstad</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Missouri (État)</region></placeName><wicri:cityArea>Departments of Pathology and Medicine, Washington University, St. Louis</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">The Journal of Protozoology</title><title level="j" type="alt">THE JOURNAL OF PROTOZOOLOGY</title><idno type="ISSN">0022-3921</idno><idno type="eISSN">1550-7408</idno><imprint><biblScope unit="vol">33</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="478">478</biblScope><biblScope unit="page" to="486">486</biblScope><biblScope unit="page-count">9</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1986-11">1986-11</date></imprint><idno type="ISSN">0022-3921</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-3921</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acid vesicles</term><term>Ameba</term><term>Amebic</term><term>Amebic adherence</term><term>Amebic cytolysis</term><term>Amebic vesicle</term><term>Ammonium</term><term>Assay</term><term>Baseline</term><term>Capracanis</term><term>Cell monolayers</term><term>Chloroquine</term><term>Coli</term><term>Control medium</term><term>Control test medium</term><term>Cycloheximide</term><term>Cytochalasin</term><term>Cytolysis</term><term>Cytolytic</term><term>Cytotoxicity studies</term><term>Dextran</term><term>Endocytic vesicles</term><term>Entamoeba</term><term>Entamoeba histolytica</term><term>Glass chips</term><term>Histolytica</term><term>Human neutrophils</term><term>Intracellular</term><term>Lectin</term><term>Lysosomal</term><term>Lysosomal enzymes</term><term>Macrophage</term><term>Mammalian cells</term><term>Mammalian target cells</term><term>Meronts</term><term>Neutrophil</term><term>Parasite</term><term>Pellet</term><term>Phagocytic events</term><term>Preincubation</term><term>Primaquine</term><term>Protein synthesis</term><term>Ravdin</term><term>Receptor</term><term>Rosette</term><term>Standard curve</term><term>Supernatant fluid</term><term>Surface lectin</term><term>Target cells</term><term>Test conditions</term><term>Test medium</term><term>Trichloroacetic material</term><term>Trophozoite</term><term>Trypan</term><term>Vascular phase</term><term>Vesicle</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">ABSTRACT. Entamoeba histolytica kills mammalian target cells in a multi‐step sequential process with separate adherence, cytolytic, and phagocytic events. In the studies reported here, we used fluorescein isothiocyanate linked to dextran to label the endocytic vesicles of the HM1 strain of E. histolytica and measure vesicle pH (5.1 ± 0.2 by spectrofluorimetry). Concentrations of NH4Cl (1.0–10.0 mM) sufficient to increase vesicle pH to °5.7 inhibited amebic killing of target Chinese hamster ovary (CHO) cells as assayed by trypan blue staining, by the release of 3H‐thymidine previously incorporated into CHO cell monolayers, and by the release of 111indium oxine from radiolabeled CHO cells. Similar effects were also observed with two other weak bases, primaquine and chloroquine (both 50 μM). In contrast, NH4Cl (10 mM) did not affect either the adherence or phagocytic events, as measured by amebic adherence to CHO cells at 4°C and by the binding and ingestion of 3H‐leucine‐labeled bacteria. In the presence of NH4Cl and and the carbohydrate ligand asialofetuin, there was no evidence of intracellular trapping of the amebic galactose‐inhibitable lectin; inhibition of adherence by cycloheximide (10 μg/ml for 3 h) suggested rapid turnover of the surface lectin. Prolonged exposure to NH4Cl for 48 h (which had no effect on amebic protein synthesis) or shorter exposure to cycloheximide (10 μg for 3 h) produced persistent inhibition of cytolysis. These results indicate that an uninterrupted acid pH in intracellular endocytic vesicles is necessary for the cytolysis of target cells by E. histolytica trophozoites.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Missouri (État)</li><li>Virginie</li></region></list><tree><country name="États-Unis"><region name="Virginie"><name sortKey="Ravdin, Jonathan I" sort="Ravdin, Jonathan I" uniqKey="Ravdin J" first="Jonathan I." last="Ravdin">Jonathan I. Ravdin</name></region><name sortKey="Gluzman, Ilya Y" sort="Gluzman, Ilya Y" uniqKey="Gluzman I" first="Ilya Y." last="Gluzman">Ilya Y. Gluzman</name><name sortKey="Krogstad, Donald J" sort="Krogstad, Donald J" uniqKey="Krogstad D" first="Donald J." last="Krogstad">Donald J. Krogstad</name><name sortKey="Murphy, Cheryl F" sort="Murphy, Cheryl F" uniqKey="Murphy C" first="Cheryl F." last="Murphy">Cheryl F. Murphy</name><name sortKey="Ravdin, Jonathan I" sort="Ravdin, Jonathan I" uniqKey="Ravdin J" first="Jonathan I." last="Ravdin">Jonathan I. Ravdin</name><name sortKey="Schlesinger, Paul H" sort="Schlesinger, Paul H" uniqKey="Schlesinger P" first="Paul H." last="Schlesinger">Paul H. Schlesinger</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003442 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003442 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:473566D72FFE98CCAD7462F82FCA9884F1ECC1D0
|texte= Acid Intracellular Vesicles and the Cytolysis of Mammalian Target Cells by Entamoeba histolytica Trophozoites
}}
| This area was generated with Dilib version V0.6.33. |  |